av A Lehrman · 2014 · Citerat av 4 — 21. Avels- och förädlingsmetoder. 21. Djuravel. 21. Avelsmål. 21. Avelsvärde Konstruerade mikro-RNA kan användas för att ändra uttrycket av specifika gener.

MicroRNA-21 (miR-21) expression is activated in multiple types of cancers, such as breast, liver, brain, prostate, myometrial cancers but also in cardiovascular diseases. MiR-21 regulates a plethora of target proteins which are involved in cellular survival, apoptosis and cell invasiveness.

Here, we developed a microRNA-21-5p delivery system using functionalized mesoporous silica nanoparticles (MSNs) with additional intrinsic therapeutic effects. These nanocarriers were encapsulated into an injectable hydrogel matrix (Gel@MSN/miR-21-5p) to enable controlled on-demand microRNA-21 delivery triggered by the local acidic microenvironment. By scanning the strip with a portable SERS reader, the sensitive quantification of microRNA-21 was realized with a detection limit as low as 84 fM. The proposed strategy was employed to detect the target in a serum sample, demonstrating its great potential in amplified point-of-care biosensing for clinical diagnosis. 2020-03-27 · Background Growing studies have focused on the role of microRNA-21 (miR-21) in glioma, thus our objective was to discuss the effect of M2 bone marrow-derived macrophage (BMDM)-derived exosomes (BMDM-Exos) shuffle miR-21 on biological functions of glioma cells by regulating paternally expressed gene 3 (PEG3).

The proposed strategy was employed to detect the target in a serum sample, demonstrating its great potential in amplified point-of … 2017-10-15 2017-07-19 2020-03-27 2020-10-26 The identification of small molecules that bind to and perturb the function of microRNAs is an attractive approach for the treatment for microRNA-associated pathologies. However, there are only a few small molecules known to interact directly with microRNAs. Here, we report the use of a small molecule microarray (SMM) screening approach to identify low molecular weight compounds that directly MicroRNA-21 (miRNA-21) is a significant biomarker which is closely related to some kinds of diseases, such as cancer, cardiovascular disease and kidney disease. Therefore, the detection of miRNA-21 is of great importance and can provide essential information … DNA mutation-induced activation of RAS-BRAF-MEK-ERK signaling associated with intermittent or chronic ultraviolet (UV) irradiation cannot exclusively explain the excessive increase of malignant melanoma (MM) incidence since the 1950s.

We previously demonstrated microRNA-21 has potential inhibitory effect on the MAP2K3 which locates upstream of P38 MAPK and was upregulated in mouse hearts upon CVB3 infection. However, the effect and underlying mechanism of miRNA-21 on CVB3 infection remain unclear. Methods We 2020-08-14 · To pursue the mechanism underlying miR-21 enhanced mitochondrial translation, we performed RNA coimmunoprecipitation with anti-Ago2, which has been previously shown to have a fraction localized in the mitochondria.

av D Pullirsch · 2010 · Citerat av 72 — P21 in the brain.37 Thus, editing seems to be especially important in the adult micro RNA and siRNA pathways in Drosophila.32 Similar func-.

In this work, we studied the roles of microRNA-21 (miR-21) in periodontitis. MiR-21 is up-regulated in both periodontitis patients and the mice that induced with periodontitis. Wu H, Ng R, Chen X, Steer CJ and Song G: MicroRNA-21 is a potential link between non-alcoholic fatty liver disease and hepatocellular carcinoma via modulation of the HBP1-p53-Srebp1c pathway. Gut gutjnl-2014-308430.

In particular, microRNA 21 (miR‐21) is upregulated in inflammatory environment and reported to induce anti‐inflammatory responses. However, the involvement of miR‐21 in pulpal inflammation and the precise mechanisms of anti‐inflammatory reactions induced by miR‐21 remain unclear.

Therefore, targeting the miR-21–Spry1 axis may be a promising therapeutic option for ameliorating post-MI cardiac fibrosis. Keywords: cardiac fibrosis, myocardial infarction, microRNA-21, Sprouty1, ERK/TGF-β/Smad signaling pathway 2018-07-23 2013-07-29 MicroRNAs are small noncoding RNA molecules that control expression of target genes. Our previous studies show that mir-21 is overexpressed in tumor tissues compared with the matched normal tissues. Moreover, suppression of mir-21 by antisense oligonucleotides inhibits tumor cell growth both in vitro and in vivo.

MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer. Oncogene. 2008;27(15):2128–2136. 2020-08-10 · Inhibition of miR-21 by LNA-anti-miR-21 was determined using RT microRNA real-time PCR. Nine days after the first LNA-anti-miR-21 injection, total RNA extraction was performed using GeneJET RNA purification kit (Thermo Scientific, Lithuania) according to the instructions. 2013-10-10 · microRNA 21 (miR-21) has been demonstrated to be significantly elevated in many types of cancers, including the hepatocellular carcinoma (HCC). In the present study, we investigated the role of miR-21 in HCC by identifying its novel targets, as well as its underlying molecular mechanism.
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blood samples, early detection, micro-RNA, miRNA, pancreatic cancer Tillgänglig från: 2016-11-21 Skapad: 2016-11-21 Senast uppdaterad: 30 okt.

MiRNA-21 (MIR21) has been shown to elicit posttranscriptional gene regulation in several tissues associated with rapid cell proliferation in addition to demonstrating anti-apoptotic features through interactions with PDCD4 mRNA 2013-07-29 · MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res. 65, 6029–6033. Crossref, Medline, Google Scholar; 25 Chen Y. Liu W. Chao T. Zhang Y. Yan X. Gong Y. Qiang B. Yuan J. Sun M. Peng X. 2008 MicroRNA-21 down-regulates the expression of tumor suppressor PDCD4 in human glioblastoma cell T98G Cancer Lett. 272197205.
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2010-05-01

AU - Carrera-Bastos, Pedro. AU - Schmitz, Gerd. PY - 2020.

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By scanning the strip with a portable SERS reader, the sensitive quantification of microRNA-21 was realized with a detection limit as low as 84 fM. The proposed strategy was employed to detect the target in a serum sample, demonstrating its great potential in amplified point-of-care biosensing for clinical diagnosis.

However, there are only a few small molecules known to interact directly with microRNAs. Here, we report the use of a small molecule microarray (SMM) screening approach to identify low molecular weight compounds that directly 2019-10-04 · Background The P38 mitogen-activated protein kinase (MAPK) pathway plays an essential role in CVB3-induced diseases. We previously demonstrated microRNA-21 has potential inhibitory effect on the MAP2K3 which locates upstream of P38 MAPK and was upregulated in mouse hearts upon CVB3 infection. However, the effect and underlying mechanism of miRNA-21 on CVB3 infection remain unclear. Methods We 2020-08-14 · To pursue the mechanism underlying miR-21 enhanced mitochondrial translation, we performed RNA coimmunoprecipitation with anti-Ago2, which has been previously shown to have a fraction localized in the mitochondria. 15 We found that Ago2 showed increased association with the Cytb mRNA after miR-21 transfection , consistent with miR-21-guided targeting of the microRNA machinery to Cytb.